As copied from the syllabus in Winter Term 2018-2019
This course will introduce you to many of the basic lab techniques used by molecular biologists. In the laboratory, you will work in teams to master basic molecular lab techniques and engage in a quarter-long project of cloning and sequencing genes in the lux operon from the bioluminescent bacterium Aliivibrio fischeri in order to induce virulence in the pathogenic bacterium Salmonella paratyphimurium. Techniques covered include: isolation of chromosomal DNA, restriction digestion, gel electrophoresis, ligation, transformation, β-gal screening of clones, plasmid mini-preps, polymerase chain reaction (PCR), sequence analysis, RNA extraction, reverse transcription (RT), and real time PCR (qPCR). Given that this is an introductory course, mastery of the techniques in the laboratory will be sought after but not required. These techniques will be vital for more advanced courses offered in the department and this course will provide you with a basic understanding of the tools used to study a variety of biological processes. This is the first exposure to some of these tools and, therefore, mastery of the techniques is not a requirement. However, an in-depth understanding of how these techniques work will be the goal for the course.