From Course Catalog: Designed to familiarize student with laboratory techniques utilized in molecular biology, specifically DNA isolation, characterization, and manipulation. Students work in teams to collect and analyze data and explain results in laboratory reports. Weekly recitations preview and review theory and techniques used in the lab. Some or all pre-requisites may be taken as either a pre-requisite or co-requisite. Please see the department for more information.
Course Rationale and Description (from syllabus): This course will introduce you to many of the basic lab techniques used by molecular biologists. Throughout the course, you will work in teams to better understand basic molecular lab techniques and engage in a quarter-long project of cloning and sequencing genes in the lux operon from the bioluminescent bacterium Aliivibrio fischeri in order to induce changes in gene expression for the bacterium Escherichia coli. Techniques covered include: isolation of chromosomal DNA, restriction digestion, gel electrophoresis, ligation, transformation, β-gal screening of clones, plasmid mini-preps, polymerase chain reaction (PCR), sequence analysis, RNA extraction, reverse transcription (RT), and real time PCR (qPCR). These techniques will be vital for more advanced courses offered in the department and this course will provide you with a basic understanding of the tools used to study a variety of biological processes. The main objective of this course is an in-depth understanding of how these techniques work.
Course Purpose (from syllabus): The Bio 219 course is a required course for BIO majors and is also required for several concentration tracks for BME majors. For BIO and BME students, this is a foundation course taken early in their curriculum that introduces students to techniques in molecular biology that will be utilized throughout their career. It also reinforces critical concepts of the Central Dogma of Biology that many other courses in the curriculum build on. The course also serves as a writing intensive course within the curriculum for both majors. Written communication skills in the sciences are critical and the Bio 219 course is the first introduction for most of the students to engage in how science is communicated in text. The course is also an early exposure for the students to learn to utilize the primary literature and to develop critical thinking and vital technical and analytical skills.