Zeal Jinwala1, David Haruch2, Swathi Swaminathan2, Alisa Morss Clyne2 1Department of Biology, Drexel University, Philadelphia 19104, 2Department of Mechanical Engineering and Mechanics, Drexel University, Philadelphia 19104
Harvard National Collegiate Research Conference- Cambridge, 2019
Endothelial cells (ECs) line the interior surface of blood vessels and drive angiogenesis, the process of new blood vessel formation from existing blood vessels. Angiogenesis is thought to be a critical factor in tumor growth [1]. Angiogenesis occurs through a complex, 3D process of endothelial proliferation, migration, and eventual maturation into a blood vessel. Recently, EC metabolic activity has been shown to be important to angiogenic sprouting [2]. For this project, we decided to test two metabolic inhibitors, namely 3PO and OGTi, in an endothelial tube formation assay to determine their effects on angiogenesis. 3PO is known to inhibit glycolytic activity, and OGTi decreases protein O-GlcNAcylation through the hexosamine biosynthetic pathway. The goal of this study was to evaluate the effect of these two inhibitors and understand the use of these inhibitory mechanisms in angiogenic sprouting in ECs.
Harvard National Collegiate Research Conference- Cambridge, 2019
Endothelial cells (ECs) line the interior surface of blood vessels and drive angiogenesis, the process of new blood vessel formation from existing blood vessels. Angiogenesis is thought to be a critical factor in tumor growth [1]. Angiogenesis occurs through a complex, 3D process of endothelial proliferation, migration, and eventual maturation into a blood vessel. Recently, EC metabolic activity has been shown to be important to angiogenic sprouting [2]. For this project, we decided to test two metabolic inhibitors, namely 3PO and OGTi, in an endothelial tube formation assay to determine their effects on angiogenesis. 3PO is known to inhibit glycolytic activity, and OGTi decreases protein O-GlcNAcylation through the hexosamine biosynthetic pathway. The goal of this study was to evaluate the effect of these two inhibitors and understand the use of these inhibitory mechanisms in angiogenic sprouting in ECs.